Reaction progress was followed using an Agilent diode array UV-visible spectrometer with Agilent ChemStation software. As the reaction progressed, the solution changed color to orange—brown Partitioning of reaction product was determined by measuring the absorbance in each phase. After mixing, phases were separated by centrifugation. The concentration of product in each phase was calculated by measuring absorbance of three samples for each phase using an Agilent diode array UV-visible spectrometer with Agilent ChemStation software.
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The identification of the major contaminants proteins and their relative concentration depicted in the tridimensional graph represents the first stage in the selection of better strategies for the purification of products or the selection of a potential host in the development of bioprocesses. Aguilar, O. Processing of soybeans Glycine max.
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